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. 2018 Dec 14;9:5316. doi: 10.1038/s41467-018-07653-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Glycans provide structural support for a giant β-barrel. Molecular model for the open conformation as viewed from within the β-barrel (a) and from the membrane (b). N-linked glycans (green) are shown as sticks (C9:N215, N256, N394; C8β:N189; and C8α:N407). Cα carbons of O-linked C9:T427 are represented as green spheres. Backbone trace of C9 C-terminus (res 522:535) is shown as cyan sticks. Disulfide bonds within MACPF domains are red and indicated by an arrow. Cα carbons of aromatic residues near the membrane (TM) are magenta spheres. c Reference-free 2D class averages of negatively stained MAC assembled from de-glycosylated C8 and C9 (bottom panel) show distorted pores on lipid monolayers as compared to native MAC (top panel). Classes are derived from similar particle numbers to enable a more direct comparison of curvature distortion (native, 706; de-glycosylated, 711). Native MAC particles are a randomly chosen subset of the data shown in Supplementary Fig. 2a which include variations across open and closed conformations. Percentage of particles belonging to each class is indicated. Scale bar, 50 Å