Fig. 5.

BMDECs in the papillomas and dysplasia are proliferating. a BrdU-positive BMDECs (arrowheads, white box area is magnified) are identified in the outer root sheath area of a deregulated HF under the tumor mass. b BMDECs in epithelium of papilloma (a serial section of Fig. 3a). b-I A group of Ki67-expressing BMDECs (area of yellow broken line and arrowheads) are identified in the basal epithelium of the papilloma. b-II Cytoplasmic beta-catenin expression was detected from BMDECs (areas of red broken line) in the epithelium of the papilloma. c BMDECs in deregulated HF structure under the tumor mass (a serial section of Fig. 3i). c-I Few Ki67-positive BMDCs (arrowheads) were identified in the section and c-II BMDCs in this area show membrane beta-catenin expression (GFP-channel in white box shows membrane expression). d BMDECs in tumor-adjacent hyperplastic epidermis (a serial section of Fig. 3f). d-I Ki67-expressing BMDCs (arrowheads) are located in HF and d-II strong membrane beta-catenin expression is detected overall in the epidermis, but low levels of cytoplasmic beta-catenin expression are detected in BMDCs in the HF (GFP-channel in white box). e–i BMDECs in dysplastic skin. e BrdU-positive BMDCs (arrowheads) are identified in the basal region of dysplastic epithelium. White box area is magnified. f f-I Ki67-expressing BMDCs (arrowheads) are identified in the basal region of dysplastic epithelium. f-II Higher magnification micrograph from different dysplastic skin sample. g Small Ki67-expressing BMDCs (white box area is magnified) are detected in the neck region (white line, located at junction between down-growing deregulated HF and interfollicular epidermis). h KCs and BMDCs in dysplastic epidermis (white boxes, magnified in h-I and h-III) show cytoplasmic beta-catenin expression, but cells in normal epidermis (yellow boxes, magnified in h-II and h-IV, orange broken line indicates basal layer of epidermis) show membrane expression. i BMDCs in dysplasia (white box area and area of white broken line in i-I) show cytoplasmic beta-catenin expression. *White scale bar, 50 µm