Figure 2.
INSATG>ATA Stem Cells Differentiate to Endocrine Cells without Insulin
(A) Schematic of in vitro β cell differentiation. Markers for specific stages of differentiation are indicated. DE, definitive endoderm; PP, pancreatic progenitors.
(B) Quantification results of cells immune-positive for both SOX17 and FOXA2 on day 4. n = 3 independent experiments. hiPSC, human iPSC.
(C) Quantification results of cells immune-positive for both PDX1 and NKX6.1 on day 12. n = 3 independent experiments.
(D) Immunostaining results for C-peptide (C-PEP) and chromogranin (CHGA) on day 27 after differentiation. Scale bar, 50 μm.
(E) Quantification results of cells immune-positive for both C-peptide and CHGA on day 27 after differentiation. n = 3 independent experiments. Data represent mean ± SD (∗∗∗p < 0.001).
(F–I) Immunostaining results for glucagon (GLUC) (F), PDX1 (G), NKX6.1 (H), and MAFA (I), co-stained with C-peptide in INS mutant and corrected cells. Scale bar, 50 μm.
(J) Quantification results of cells immune-positive for CHGA, MAFA, NKX6.1, PDX1, and GLUC in INS mutant and corrected cells. n = 3 independent experiments. Significance was tested by Student’s t test.
See also Figure S2.