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. 2018 Nov 29;11(6):1407–1415. doi: 10.1016/j.stemcr.2018.11.006

Figure 3.

Figure 3

Gene Correction Restores Insulin Expression and Insulin mRNA Stability and Increases Spliced XBP-1

(A) INS mRNA quantitation results by qRT-PCR for INS mutant β-like cells in comparison with corrected and wild-type cells. n = 3 independent experiments. Data represent mean ± SD (∗∗∗p < 0.001). hESC, human ESC; TBP, TATA-box binding protein; WT, wild-type.

(B) Agarose gel electrophoresis results for insulin mRNA after qRT-PCR.

(C) Spliced XBP1 (sXBP1) mRNA quantitation results by qRT-PCR for INS mutant β-like cells in comparison with corrected and wild-type cells. n = 3 independent experiments. Data represent mean ± SD (∗∗∗p < 0.001).

(D) RT-PCR results for XBP1 and sXBP1 mRNA using agarose gel electrophoresis.

(E) Analysis of insulin content using ELISA for INS mutant, corrected, and WT β-like cells. n = 3 independent experiments.

(F) Analysis of pro-insulin content using ELISA for INS mutant, corrected, and WT β-like cells. n = 3 independent experiments.

(G) Analysis of insulin secretion in INS mutant and corrected β-like cells using ELISA. Insulin secretion was analyzed under 3.3 mM glucose and 30.5 mM KCl conditions. n = 3 independent experiments. Significance was tested by Student’s t test.