Fig. 4.

BDNF inhibited the increased expression of PUMA induced by etoposide. a, b TB3 and TB8 cells were pre-treated with BDNF (100 ng/ml) for 1 h, followed by treatment with etoposide (0.5 μg/ml) for 8 h, and cells were harvested. The expression of PUMA was detected by Western blot (a) and RT-PCR (b). Bars, SD P-value were tested by Student’s t test. **P < 0.01. c The expression of PUMA was detected by Western blot after transfection of P53 siRNA into TB3 and TB8 cells