Fig. 6.

BDNF protected TrkB-expressing TB3 and TB8 cells from over-expression PUMA induced cell death. TrkB-expressing TB3 and TB8 cells were infected with activated PUMA lentiviral or empty vector, 24 h after infection, BDNF (100 ng/ml) was added. Treated with BDNF for 3 h, cells were harvested, and the expression of PUMA was tested by Western blot and RT-PCR (a). The cell survival was tested by MTS assay after treated with BDNF for 48 h (b). Bars, SD P-value were tested by Student’s t test. **P < 0.01. c After treated PUMA-lentiviral-infected TB3 and TB8 cells with BDNF (100 ng/ml), real-time evaluation of cell confluence was performed by IncuCyte ZOOM. The points showed three replicates. Bars, SD P-value were tested by Student’s t test. **P < 0.01