KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-GFP | Clontech | JL-8 |
| Mouse anti-Flag M2 antibody | Sigma Aldrich | F3165 |
| Bacterial and Virus Strains | ||
| E. coli BL21 (DE3) | NEB | C2527I |
| E. coli DH5α | NEB | C2987I |
| E. coli DH10Bac | Life Technologies | 10361012 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Pierce™ Glutathione Agarose | Thermo Scientific | 16101 |
| Q Sepharose™ High Performance | GE Healthcare | 17-1014-01 |
| Superdex 200 Increase 10/300 GL | GE Healthcare | 28-9909-44 |
| TEV protease | In house | N/A |
| SelK, SelS, USP1 peptides | Bio-Synthesis, Inc | N/A |
| SelK 3aa peptide | GenScript | N/A |
| AlphaScreen™ Streptavidin-coated donor beads | PerkinElmer | 6760002S |
| AlphaLISA™ anti-GST AlphaScreen acceptor beads | PerkinElmer | AL110C |
| Cesium Iodide | MP Biomedicals | 21004 |
| Ammonium Acetate | Sigma-Aldrich | A7262 |
| ANTI-FLAG M2 Affinity Gel | Sigma-Aldrich | A2220 |
| Streptavidin Biosensors | Pall Life Sciences/ForteBio | 18-5019 |
| Critical Commercial Assays | ||
| Amino acid analysis | TAMU Protein Chemistry Lab | N/A |
| Deposited Data | ||
| KLHDC2-SelK | This paper | 6DO3 |
| KLHDC2-SelS | This paper | 6DO4 |
| KLHDC2-USP1 | This paper | 6DO5 |
| Experimental Models: Cell Lines | ||
| Spodoptera frugiperda: Sf9 | Life Technologies | B825-01 |
| Trichoplusia ni: HighFive | Life Technologies | B85502 |
| Human: HEK293T | ATCC | CRL-3216 |
| Recombinant DNA | ||
| pFB-GST-KLHDC2(1-362) | This paper | N/A |
| pFB-GST-KLHDC2 K147A | This paper | N/A |
| pFB-GST-KLHDC2 R189A | This paper | N/A |
| pFB-GST-KLHDC2 A219L | This paper | N/A |
| pFB-GST-KLHDC2 A220L | This paper | N/A |
| pFB-GST-KLHDC2 R236A | This paper | N/A |
| pFB-GST-KLHDC2 R236E | This paper | N/A |
| pFB-GST-KLHDC2 R241A | This paper | N/A |
| pFB-GST-KLHDC2 R241E | This paper | N/A |
| pFB-GST-KLHDC2 R241K | This paper | N/A |
| pFB-GST-KLHDC2 R241L | This paper | N/A |
| pFB-GST-KLHDC2 S269A | This paper | N/A |
| pFB-GST-KLHDC2 S269E | This paper | N/A |
| pFB-GST-KLHDC2 S269L | This paper | N/A |
| pGEX SelK 8aa | This paper | N/A |
| pGEX SelK 12aa | This paper | N/A |
| pLenti-GPS | Emanuele et al., 2011 | N/A |
| Software and Algorithms | ||
| Phenix | Adams et al., 2002 | https://www.phenix-online.org/ |
| Coot | Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| HKL2000 | Otwinowski and Minor, 1997 | http://www.hkl-xray.com/ |
| PyMOL | Pymol | https://pymol.org/2/ |
| Prism 4 | GraphPad | N/A |
| FlowJo | FLOWJO | N/A |
| ImageJ | NIH | N/A |
| UCSF Chimera | Pettersen et al., 2004 | https://www.cgl.ucsf.edu/chimera/ |
| Data Analysis V 9.0 | Pall Life Sciences/ForteBio | N/A |
| MassLynx v. 4.1 | Waters Corporation | https://www.waters.com |
| LigPlot+ | Laskowski and Swindells (2011) | https://www.ebi.ac.uk/thornton-srv/software/LigPlus/ |
| Other | ||
| EnSpire reader | PerkinElmer | N/A |
| Octet Red 96 | Pall Life Sciences/ForteBio | N/A |
| Modified Synapt G2 HDMS Time-of-Flight mass spectrometer | Waters Corporation |
https://www.waters.com
DOI: 10.1039/C5AN02107C |
| Model P-97 Micropipette Puller | Sutter Instrument | https://www.sutter.com |
| Borosilicate capillaries 1.0 mm OD, 0.78 mm ID, 150 mm L | Harvard Apparatus | 30-0035 |
| LSR Fortessa | BD Biosciences | N/A |