Fig 4. Intensity-weighted average lifetime analysis as a function of bilayer vesicle composition.
POPC bilayer vesicles (50 μM) contained 10 mol% fully saturated GlcCer of different N-linked acyl chain length and 1 mol % trans-parinaric acid. The trans-parinaric acid (TPA) lifetime is short in fluid membranes (<5 ns), intermediate in a liquid-ordered phases, and long (35 ns) in gel and solid membranes. Average lifetimes (ns) are shown for each composition, with average ± SD for at least three independent measurements.