Skip to main content
. 2018 Dec 14;13(12):e0209230. doi: 10.1371/journal.pone.0209230

Fig 6. GlcCer dependent GLTP and VAP-A binding.

Fig 6

Pull-down assays with subsequent Western blott analysis of GLTP with bound GlcCer and VAP-A. The GST-tagged VAP-A was pulled-down using glutathione sepharose beads and washed, carrying along any bound GLTP. The upper blot is anti-GLTP and the lower blot anti-GST antibodies. In the lane indicated with CTRL only GLTP and VAP-A was pulled down, the lane indicated POPC contains GLTP, POPC and VAP-A. The blot shown is representative of three different independent experiments.