Fig 2. Reconstitution of human leukemia with distinct expression of CD25 at the primary and secondary transplantations in a PDX model.

Lin^–CD34⁺CD38^– cells from AML01 (A), 02 (B), and 03 (C) were sorted into CD25-positive and -negative populations, and then transplanted to primary recipients. Expression of CD25 on engrafted Lin^–CD34⁺ cells was analyzed. Engrafted BM cells from AML01 and 02 were sorted again into CD25-positive and -negative populations, and then transplanted into secondary recipients. Expression of CD25 and CD38 on engrafted Lin^–CD34⁺ cells in secondary recipients was analyzed.