Fig 3. Reconstitution of human leukemia with distinct expression of CD25 at the primary transplantation into mice, with failure at the secondary transplantation.

Lin^–CD34⁺CD38^– cells from AML04 (A), 05 (B), and 06 (C) were sorted into CD25-positive and -negative populations, and then transplanted to primary recipients. Expression of CD3/CD19, CD33, CD34, CD38, and CD25 on engrafted cells was analyzed. The hCD45⁺ cells were sorted and transplanted to secondary recipients. No engraftment was observed in mice transplanted with hCD45⁺ cells from AML04, 05, or 06.