Skip to main content
. 2018 Dec 14;13(12):e0208777. doi: 10.1371/journal.pone.0208777

Fig 5. Effect of miR-2110 over-expression and TSKU knockdown on cell differentiation and survival in Kelly, CHLA-90 and SKNFI cells.

Fig 5

The indicated cell lines were transfected with as above, and neurite outgrowth, cell confluence, cell viability and protein expression levels were measured as above. A, Cell images showing that miR-2110 mimic and siTSKUs did not induce neurite outgrowth in the three cell lines. B, Time-dependent cell confluence change after each treatment. *, p < 0.05 for all three treatments (miR-2110, siTSKU-1 and siTSKU-2) compared to the control at the respective time points. (C) Cell viability associated with each treatment. *, p < 0.05 compared to control. (D) Effect of miR-2110 mimic and siTSKU-1 on protein expression of cell differentiation markers (βIII-tubulin, GAP43, NSE), apoptosis marker (cleaved PARP) and TSKU, with calnexin measured as a loading control. (E) Effect of siTSKUs and miR-2110 mimic on caspase 3/7 activity. *, p < 0.05 compared to control.