Fig 4. Immuno-histochemical staining for basal markers in tumor transplants generated from Cd²⁺ -transformed cell line (Cd#3).

(A). CDH3. There is no staining in the differentiated (*) or the less differentiates areas (#) of the tumor transplants. (B). CD44. There is strong membranous staining for CD44 in the less differentiated cells (#) located at the periphery of the tumor nests, whereas the well-differentiated cells (*) located in the center of the tumor nests show weak or no staining for CD44. (C). KRT1. The well-differentiated cells (*) in the center of tumor nests are strongly positive for KRT1, whereas the peripheral less differentiated cells (#) show weaker staining of KRT1. (D). KRT5. The staining for KRT5 is diffuse with strong staining in the differentiated (*) as well as less differentiated (#) areas of the tumor. (E). KRT6. The staining for KRT6 is strong in the well-differentiated cells (*) located in the center of the tumor nests, whereas the staining is weak to absent in the less differentiated cells (#) located at the periphery of the tumor nests. (F). KRT14. The well-differentiated cells (*) in the center of the tumor nests show weak or no staining for KRT14, whereas the less differentiated peripheral cells (#) are strongly positive for KRT14. (G). KRT16. The staining for KRT16 is strong in the well-differentiated cells (*) located in the center of the tumor nests, whereas the staining is absent in the less differentiated cells (#) located at the periphery of the tumor nests. (H). KRT17. The staining for KRT17 is strong in the less differentiated cells (#) located at the periphery of the tumor nests while the center of the tumor nests containing well-differentiated cells are weakly positive (*). The brown color indicates the presence of the protein whereas the blue/purple color indicates the nuclei that are stained with the counterstain hematoxylin. All images are at a magnification of 200X.