Fig 7. Gene expression analysis of basal and luminal marker genes in the As³⁺ and Cd²⁺-transformed cell lines and their corresponding urospheres.

(A and B). Real-time PCR analysis of basal marker genes in Cd²⁺ (A) and As³⁺ (B) transformed cell lines and urospheres. (C and D). Real-time PCR analysis of luminal marker genes in Cd²⁺ (C) and As³⁺ (D)-transformed cell lines and urospheres. Analysis was done in triplicates and is plotted as the mean ± SD. The data is plotted on a log base 2 scale per transcript of β-actin times 1000. * indicates significantly different at p < 0.05 from transformed UROtsa cells. # indicates significantly different at p < 0.01 from transformed UROtsa cells. ^ indicates significantly different at p < 0.001 from transformed UROtsa cells.