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. 2018 Dec 14;13(12):e0207877. doi: 10.1371/journal.pone.0207877

Fig 8. Histology and Immuno-histochemical staining of nodules formed by UROtsa cells injected with matrigel in immune compromised mice.

Fig 8

(A). Histology of UROtsa nodule at lower magnification (100X). Epithelial nests in different size and shape and single cells seen in the matrigel (arrows). Spindle stroma cells are present in the right part of the image (arrowhead), mainly in the right part of the image. (B). Histology of UROtsa matrigel nodule at higher magnification (200X). Most of the epithelial nests are irregular in shape with branches or angular formation. A few of epithelial nests are oval or round in shape, similar to the von Brunn’s nests (arrow) in human bladder. Some smaller nests or clusters are composed of only a few epithelial cells. (C). Immunostaining for KRT5. The epithelial nests of various size and some single cells are strongly positive for KRT5 (arrow). The spindle stroma cells are negative for KRT5 (arrowhead). (D). Immunostaining for KRT6. The epithelial nests and single epithelial cells are negative for KRT6 (arrows). All images are at a magnification of 200X unless indicated.