Skip to main content
. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Cancer Res. 2018 May 14;78(14):4007–4021. doi: 10.1158/0008-5472.CAN-17-3691

Figure 2.

Figure 2.

CUDC-907 induces G1 cell-cycle arrest, CDKN1A/p21 expression, and apoptosis. A, Cell-cycle distribution of SF188 (pHGG) and SF8628 (DIPG) following CUDC-907 (2.5 or 5.0 nmol/L) for 16 hours with or without irradiation (4 Gy); all samples were fixed 6 hours after irradiation. B and C, Protein levels of cell-cycle regulators p21 and p-CDK1/2 (B) and 18S rRNA normalized mRNA expression of p21 (C), 16 hours after CUDC-907 (100 nmol/L), irradiation (4 Gy) or combination (4 Gy 16 hours after CUDC-907). D and E, Enrichment of H3K9 acetylation (D) and RNA Pol II recruitment (E) to the promoter and in the body of the p21 gene in SF188 cells. F, SF188 and SF8628 assessed for apoptosis, measured by caspase-3/7 activity, 48 hours after CUDC-907 (1.25 nmol/L), irradiation (4 Gy), or combination (4 Gy administered 16 hours after CUDC-907). Values are mean ± SEM, normalized to control (DMSO) and only P < 0.05 are shown (n = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).