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. Author manuscript; available in PMC: 2019 Nov 29.
Published in final edited form as: Cell. 2018 Nov 1;175(6):1651–1664.e14. doi: 10.1016/j.cell.2018.09.047

Figure 4. Caspase-11 and caspase-1 are recruited to the NLRP6 inflammasome through ASC.

Figure 4.

(A−E) Immortalized BMDMs were left uninfected or infected with Listeria for 12 h or indicated times. (F) Poly(I:C)-primed immortalized BMDMs were incubated with LTA in Opti-MEM supplemented with 0.005% saponin for 4 h. (A, D−F) The cells were lysed, immunoprecipitated (IP) and analyzed by immunoblotting (IB). (D−F) Cells were treated with DSP and DTBP before IP. Whole cell lysates are shown as the input. Asterisk, IgG. Blots of caspase-11 were cropped to reveal protein bands at different exposures. (B and C) The cells were fixed, immunostained and ASC specks (arrowheads) were counted. ASC, green; caspase-11 (Flag), red; and nuclei, blue. Scale bars, 10 µm. ND, not detected. Results are representative of at least (A, D−F) three or (B and C) two independent experiments, and error bars denote s.d. of each group. See also Figure S5.

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