Figure 5. Processed caspase-11 promotes caspase-1-mediated IL-18 secretion.
(A) Schematic representation of predictive cleavage sites in pro-caspase-11. (B−E) Immortalized Casp11−/− BMDMs reconstituted with WT or mutants of caspase-11 were infected with Listeria for 12 h. (B and C) The cell lysates (Lysate) were subjected to immunoblotting, or (D and E) the supernatants were subjected to ELISA. Blots of caspase-11 were cropped to reveal protein bands at different exposures. Results are representative of at least three independent experiments, and error bars denote s.d. of triplicate wells. **P < 0.01, ****P < 0.0001. See also Figure S5.