Figure 7. NLRP6-caspase-11 axis exacerbates Listeria infection.
Mice were infected with (A, C−H) 104 or (B) 105 cfu of Listeria intravenously. (A, F−H) The organs were removed on day 4 for cfu counting. (B) Mouse survival was monitored for 10 days. (C and The spleen homogenates were immunoblotted. (E) The sera were collected on day 4, and cytokine levels were determined by ELISA. (G) Recombinant IL-18 (rIL-18) was administrated on day 2 post-infection (P.I.). Blots of caspase-11 were cropped to reveal protein bands at different exposures. Results are (A, B, F, G) pooled from two independent experiments or (C−E, H) representative of three independent experiments, and error bars denote s.d. of each group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. See also Figures S6 and S7.