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. 2018 Nov 5;46(22):12190–12191. doi: 10.1093/nar/gky1115

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — A3B is a processive enzyme that both slides and moves 3-dimensionally on ssDNA. Processivity of A3B was tested on ssDNA substrates that contained a fluorescein-labeled deoxythymidine (yellow star) between two 5’ATC deamination motifs separated by different distances. (A) Deamination of a 60 nt ssDNA substrate with deamination motifs spaced 5 nt apart. Single deaminations of the 5’C and 3’C are detected as the appearance of labeled 42- and 23-nt fragments, respectively; double deamination of both C residues on the same molecule results in a 5 nt labeled fragment. (B) Deamination of a 69 nt ssDNA substrate with deamination motifs spaced 14 nt apart. Single deaminations of the 5’C and 3’C are detected as the appearance of labeled 51- and 32-nt fragments, respectively; double deamination of both C residues on the same molecule results in a 14 nt labeled fragment. (C) Deamination of a 118 nt ssDNA substrate with deaminated cytosines spaced 63 nt apart. Single deaminations of the 5’C and 3’C are detected as the appearance of labeled 100- and 81-nt fragments, respectively; double deamination of both C residues on the same molecule results in a 63 nt labeled fragment. (D) Deamination of a 118 nt ssDNA substrate as in (C) but with a 30 nt complementary DNA annealed between the deamination motifs. The measurements of processivity factor (P.F.) and the standard deviation (S.D.) from three independent experiments are shown below the gel.