Figure 5.

Pif1 physically interacts with PCNA via conserved PIP box. (A) Consensus PIP box (17) in comparison to identified PIP box in Pif1 and mutated PIP version (pif1-PIPmut). Consensus residues are marked in blue and mutations in red, [x] denotes spacing variations. Residues 752–761 in Pif1 are shown. (B) Schematic illustration of ELISA experimental setting for detecting the interaction between immobilized PIP peptides and PCNA. (C) Relative ELISA signal for PCNA–Pif1 PIP peptide interaction. Significance was determined by t-test. ***P< 0.0001. Error bars are ± SD, n = 3.