Figure 2.

RAP-MS identifies the insulin mRNA-binding proteins. (A) A schematic represented the multiple steps of RAP-MS. (B) Insulin mRNA-protein complexes were identified by incubating biotinylated insulin mRNA antisense probes with protein extracts from INS-1 cells. The arrows indicate seven bands that were pulled down by the biotinylated insulin mRNA antisense probes; these bands were then identified by MS. M denotes marker proteins to the left. (C) A list of insulin mRNA-associated proteins identified by RAP-MS analysis in INS-1 cells.