Table 3.

Experimental trends in thermal stability of the aptamer constructs in presence and absence of the L-Arm ligand

System	Motif	T m (°C)a	ΔTm (°C)	Change (reference)c
apt	n.a.	55.2 ± 1.6*	ref*	–
apt(ImH+)	Non	56.4 ± 1.3	1.2 ± 2.0	+ T3ImH+ (*)
f2-apt	n.a.	53.5 ± 0.7∧	f2-ref∧	–
			−1.7 ± 2.0	+ double flip (*)
+ L-Armb		62.2 ± 1.7	8.7 ± 1.9	+ L-Arm (∧)
f2-apt (ImH+)	in	64.9 ± 1.1	11.4 ± 1.3#	+ T3ImH+ (∧)
+ L-Arm[b]		69.4 ± 0.8	4.5 ± 1.4	+ L-Arm (#)
			15.9 ± 1.3	+(T3ImH+ & L-Arm) (*)

^a1 μM strand concentration, 100 mM NaCl, 10 mM sodium phosphate, pH 7.0 The reported errors are one standard deviation as described in the materials section.

^bThe L-Arm concentration used was 10 mM representing a 10 000:1 L-Arm/aptamer ratio.

^c *, ∧ and # refer to the same labels introduced in column T_m in order to identify the temperature used as a reference to gauge the impact of the change mentioned.