Figure 4.

SDOS is a RBP. (A and B) Autoradiography and WB analysis of eGFP-fused (A) and Flag-HA tagged (B) proteins after PNK assay in the presence of increasing concentration of Rnase I (panel A: 1, 10, 50, 100 ng/µl; panel B: 1, 10, 100 ng/µl). MOV10-YFP was used as positive control. Unfused eGFP and M2-Flag beads were used as negative control, respectively. Asterisks on autoradiography indicate specific bands. (C) eGFP-based RNA-binding assay with schematic representation of the procedure (left) and relative green fluorescence signal of RNA-bound fraction over input from cells expressing different eGFP fusion proteins (right). Numbers above bars indicate the statistical significance (P-value), based on one-sample t-test (n = 3). (D) BindUP RNA-binding surface prediction of SDOS (PDB ID: 3kvh). The most likely RNA-binding surfaces are represented in blue and orange, while the NUDIX domain is represented in red. (E) Electrostatic surface potential of SDOS. The protein surface is represented according to charge.