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. 2018 Sep 27;46(22):12067–12086. doi: 10.1093/nar/gky873

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — SDOS expression alters cilium formation. (A) Representative image of the primary cilium (red) and its length in HeLa cells upon overexpression of SDOS-eGFP or the respective eGFP control. Cilium formation has been induced by serum starvation (48 h), then cells have been fixed, permeabilized and hybridized with Acetyl-Tubulin as a primary cilium marker. Nuclei are stained with DAPI (blue). Scale bar: 15 μM. (B) Representative image of the primary cilium (green) and its length in HeLa cells upon induction of SDOS-directed shRNA and the respective shGFP control. Cilium formation has been induced by serum starvation (48 h), then cells have been fixed, permeabilized and hybridized with Acetyl-Tubulin as a primary cilium marker. Nuclei are stained with DAPI (blu). Scale bar: 15 μM. (C) Length of primary cilia measured by confocal microscopy analysis of Acetyl-Tubulin-stained HeLa cells upon SDOS overexpression (SDOS-eGFP, n = 146) or silencing (shSDOS, n = 110) and relative eGFP (n = 165) and shGFP (n = 168) controls. Numbers indicate the statistical significance (P-value) based on the Student’s t-test. (D) Bar graph representing the percentage of ciliated HeLa cells upon SDOS silencing (shSDOS) or overexpression (SDOS-GFP) compared to the respective shGFP and eGFP controls.