Table 2.

Binding of ARTD2 to DNA breaks measured from using SPR and FP

	SPR DNA-12 (5′-P) 300 mM NaCl						FP DNA-10 100 mM NaCl	FP DNA-10 150 mM NaCl
Proteins	K D (nM)	k a1 (M−1 s−1)	k d1 (s−1)	k a2 (s−1)	k d2 (s−1)	chi2	K D ± SE (nM)	K D ± SE (nM)
WT	83	4.2 × 104	0.008	0.002	0.002	2.76	41 ± 4.6	870 ± 200
R153A	NB						76 ± 14	1900 ± 560
W151A	NB						66 ± 14	1100 ± 340
N127A	NB						54 ± 6.1	ND
Q159A	8100	1.4 × 103	0.033	0.002	0.001	0.07	65 ± 15	770 ± 260
K183A	NB						31 ± 7.1	2200 ± 630
Y132F	250	7.9 × 104	0.02	0.007	0.001	0.06	40 ± 5.1	ND
N128A	88	1.3 × 105	0.04	0.002	0.001	0.51	77 ± 16	ND
Y201F	NB						70 ± 16	890 ± 260
K179A	NB						62 ± 17	ND
WGRCAT	940	7.2 × 103	0.01	0.001	0.001		5000 ± 2400	>8000
WT DNA-11 (no 5′-P)	66	5.1 × 104	0.015	0.005	0.001	0.06

NB, no binding detected or signal too low for analysis. ND, not determined.