Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 13;46(22):11776–11788. doi: 10.1093/nar/gky926

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Interactions of HDAC4c/-5c with SM1 mutants generated based on structural information. (A) SM1 mutant alleles obtained via OPTHiS screening (O) or site-directed mutagenesis (S) are shown in red and blue colors, respectively. SM1(S) mutant alleles were designed based on the HDAC4c-SP1 structure. (B) Yeast two-hybrid assay. LexA-fused SM1F containing indicated SM1(S) mutation was tested for B42-GBD-HDAC4c/-5c in EGY48 strain. (C) In vitro GST pull-down assay. In vitro synthesized HDAC4c/-5c were examined for their binding to GST or GST-fused SM1F derivatives. (D) Quantitative BiFC assay for the interaction between indicated KGN-SM1F mutants and KGC-HAC4c/-5c proteins in HEK293 cells. WT: wild-type, E.V: empty vector, N.T: no transfection.