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. 2018 Oct 13;46(22):11968–11979. doi: 10.1093/nar/gky932

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — SOX binds to a stretch of adenosines upstream of the cleavage site. (A) An RNA footprinting assay was carried out by incubating 5′ ³²P-labeled LIMD1-54 with RNase T1 in the presence (lanes 3–7) or absence (lane 2) of a dilution series of SOX (8–0.5 μM). Hydrolysis (–OH, lane 1) and RNase T1 (T1, lane 8) ladders of the RNA were also generated in order to map the location of protected sites. Lines on the right denote protected base pairs. (B) Diagram of LIMD1-54 indicating sites protected from RNase T1 cleavage by SOX. The upstream SOX binding site is colored orange while the protected residues surrounding the cut site are shown in red.