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Single turnover kinetics for I-LtrI AEGE and AEGD variants on the ATAC and AACC substrates.
| Protein | Substrate | Vmax (nM/s) | k cat (nmol/sec) | K m (nM) | Efficiency (kcat/Km) |
|---|---|---|---|---|---|
| AEGE | ATAC | 0.29 | 0.059 ± 0.0021 | 48 ± 10 | 0.0012 |
| AEGE | AACC | 0.29 | 0.058 ± 0.0077 | 458 ± 138 | 0.00013 |
| AEGD | ATAC | 1.06 | 0.21 ± 0.009 | 163 ± 26 | 0.0013 |
| AEGD | AACC | 0.77 | 0.16 ± 0.027 | 561 ± 178 | 0.00028 |
