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. 2018 Aug 24;46(22):e131. doi: 10.1093/nar/gky767

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Stability of eccDNAs generated by CRISPR-C. (A) Outline of time-course experiment. (B) Left, percent fluorescence cells from 1 to 6 cell passages (1:3 split-ratio) in absence of tetracycline (-Tet); right, corresponding FACS gating images. (C) As B description, cells propagated in +Tet conditions. (D) Outward PCR analysis of [EGFP^circles] at passage 1 to 6; left, -Tet; right, +Tet. Cr1+Cr2 (1+2), CRISPR gRNAs; C, control – gRNA; ctrl, DNA template control. (E) Quantification of PCR analysis by Image J, solid lines; theoretical 1/3 dilution rate, dash lines.