Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Platynereis dumerilii) |
PKD1-1 | This paper | GenBank_Acc#: MH035679 |
|
| Gene (P. dumerilii) |
PKD2-1 | This paper | GenBank_Acc#: MH035677 |
|
| Gene (P. dumerilii) |
PKD2-2 | This paper | GenBank_Acc#: MH035678 |
|
| Gene (P. dumerilii) |
LOV-1 | This paper | GenBank_Acc#: MH035680 |
|
| Strain, strain background (P. dumerilii) |
Wild Type | Tübingen cultures, Max Planck Institute for Developmental Biology |
NCBITaxon:6359 | |
| Strain, strain background (P. dumerilii) |
PKD1-1Δ1/Δ1 | This paper | Progenitor:Tübingen WT strain using PKD1-1_truT1 gRNA |
|
| Strain, strain background (P. dumerilii) |
PKD1-1i1/i1 | This paper | Progenitor:Tübingen WT strain using PKD1-1_T5 gRNA |
|
| Strain, strain background (P. dumerilii) |
PKD2-1Δ137/Δ137;
PKD2-1mut/mut |
This paper | Progenitor:Tübingen WT strain using PKD2-1_truT2 gRNA |
|
| Strain, strain background (P. dumerilii) |
PKD2-1Δ5/Δ5; PKD2-1mut/mut | This paper | Progenitor:Tübingen WT strain using PKD2-1_truT2 gRNA |
|
| Strain, strain background (P. dumerilii) |
PKD2-1Δ137/Δ5; PKD2-1mut/mut | This paper | Progenitor:PKD2-1Δ5 and PKD2-1Δ137 allele-carrying strains. |
|
| Strain, strain background (Centropages typicus) |
Wild Type | National Institute for Aquatic Resources culture, Technical University of Denmark |
NCBITaxon: 463189 |
|
| Antibody | Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 |
ThermoFisher Scientific | Cat#:A11001 | (1:250) |
| Antibody | Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 633 |
ThermoFisher Scientific |
Cat#:A21070 | (1:250) |
| Antibody | Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse |
Sigma-Aldrich | Cat#:T6793, RRID:AB_477585 |
(1:250) |
| Antibody | HA-Tag (C29F4) Rabbit mAb |
Cell Signaling Technology |
Cat#:3724P | (1:250) |
| Recombinant DNA reagent |
pUC57-PKD2-1p::Palmi- 3xHA-tdTomato (plasmid) |
This paper | Promoter construct: injected at 250 ng/μl |
|
| Recombinant DNA reagent |
pUC57-PKD1-1p::Palmi- 3xHA-tdTomato (plasmid) |
This paper | Promoter construct: injected at 250 ng/μl |
|
| Recombinant DNA reagent |
pUC57-T7-RPP2-tdTomato- P2A-GCaMP6 (plasmid) |
This paper | Used for generating tdTomato-P2A-GCaMP6s mRNA |
|
| Recombinant DNA reagent |
pMJ920 (plasmid) | DOI:10.7554/ eLife.00471 |
Addgene_plasmid: #42234 |
Laboratory of Jennifer Doudna |
| Recombinant DNA reagent |
pUC57-T7-RPP2-hSpCas9- HA-2XNLS-GFP (plasmid) |
This paper | Used for generating Cas9-GFP mRNA |
|
| Recombinant DNA reagent |
pGP-CMV-GCaMP6s (plasmid) |
DOI:10.1038/ nature12354 |
Addgene_plasmid: #40753 |
Laboratory of Douglas Kim |
| Recombinant DNA reagent |
pX335-U6-Chimeric_BB- CBh-hSpCas9n(D10A) (plasmid) |
DOI:10.1126/ science.1231143 |
Addgene_plasmid: #42335 |
Laboratory of Feng Zhang |
| Recombinant DNA reagent |
DR274 (plasmid) | DOI:10.1038/ nbt.2501 |
Addgene_plasmid: #42250 |
Laboratory of Keith Joung |
| Recombinant DNA reagent |
PKD2-1_truT2 (plasmid) | This paper | Derived from Addgene plasmid 42250.Used for gRNA synthesis |
|
| Recombinant DNA reagent |
PKD1-1_truT1 (plasmid) | This paper | Derived from Addgene plasmid 42335.Used for gRNA synthesis |
|
| Recombinant DNA reagent |
PKD1-1_T5 (plasmid) | This paper | Derived from Addgene plasmid 42335.Used for gRNA synthesis |
|
| Recombinant DNA reagent |
pCRII-PKD2-1 (plasmid) | This paper | Used for synthesizing WMISH probes |
|
| Recombinant DNA reagent |
pCRII-PKD1-1
(plasmid) |
This paper | Used for synthesizing WMISH probes |
|
| Sequence-based reagent |
PKD1-1-WMISH forward primer (5'- > 3') |
This paper | TGTCTTTGTAACTGTTGGTCTGGT | |
| Sequence-based reagent |
PKD1-1-WMISH reverse primer (5'- > 3') |
This paper | ATGTTCCTCAGAGATTCCTTCATC | |
| Sequence-based reagent |
PKD2-1 WMISH forward primer (5'- > 3') |
This paper | TCCTCGTCATCATAATATCCATTG | |
| Sequence-based reagent |
PKD2-1 WMISH reverse primer (5'- > 3') |
This paper | CTCTCTTTGTTGAGTTGGTCCTTT | |
| Sequence-based reagent |
PKD1-1 T5 (gRNA) | This paper | 20 ng/μl; AGGCGGATATCAGTGAAGCG; It generated i1 allele in PKD1-1. |
|
| Sequence-based reagent |
PKD1-1 truT1 (gRNA) | This paper | 20 ng/μl; TGTGTCAACTTTACCCCA. It generated Δ1 allele in PKD1-1. |
|
| Sequence-based reagent |
PKD2-1 truT2 (gRNA) | This paper | 20 ng/μl; GGGCCACCCGTCAGACTG. It generated Δ137 and Δ5 alleles in PKD2-1. |
|
| Sequence-based reagent |
PKD1-1_Genotyping- truT1andT5-forward primer (5'- > 3') |
This paper | TCAAACTGGTCAAGATTAAATTCCAGA | |
| Sequence-based reagent |
PKD1-1_Genotyping- truT1andT5-reverse primer (5'- > 3') |
This paper | TCTATTTCACTAATGTTGTTCCTGATG | |
| Sequence-based reagent |
PKD1-1 truT1-sequencing primer (5'- > 3') |
This paper | TAAGTGAAGGTCACATACTCGTCAGT | |
| Sequence-based reagent |
PKD1-1 T5-sequencing primer (5'- > 3') |
This paper | TATGAGACTGAATGCACAATAGAGTTT | |
| Sequence-based reagent |
PKD2-1_Genotyping-truT2- forward primer (5'- > 3') |
This paper | CCCTTTGTGAGCAGGAGATGCCCTGC | |
| Sequence-based reagent |
PKD2-1_Genotyping-truT2- reverse primer (5'- > 3') |
This paper | CATGACCTGAGTGTAGTAGTACATGGT | |
| Sequence-based reagent |
PKD2-1 truT2-sequencing primer (5'- > 3') |
This paper | AACCTTCAAATATGTTCACTACAATCC | |
| Sequence-based reagent |
BamHI-PKD1-1promoter forward primer (5'- > 3') |
This paper | ATGGATCCGGAAGCCTGATAACAAAGTGAGTAGGAA | |
| Sequence-based reagent |
AscI-PKD1-1promoter reverse primer (5'- > 3') |
This paper | AAGGCGCGCCGCTCCTCCTCCAAGTGGCTCTACCATCTCCGTCTGTATC | |
| Sequence-based reagent |
BamHI-PKD1-1promoter forward primer (5'- > 3') |
This paper | ATGGATCCAATAAAATTTGAACCGAGGCCAATGGA | |
| Sequence-based reagent |
AscI-PKD1-1promoter reverse primer (5'- > 3') |
This paper | AAGGCGCGCCGCTCCTCCTCCGGGGCGTGACATCAGCCAGTAGTTGGT | |
| Commercial assay or kit |
QuickExtract | Epicentre,US | Cat#:QE09050 | |
| Commercial assay or kit |
MEGAshortscript T7 Transcription Kit |
Ambion, ThermoFisher Scientific |
Cat#:AM1354 | |
| Commercial assay or kit |
mMESSAGE mMACHINE T7 ULTRA Transcription Kit |
Ambion, ThermoFisher Scientific |
Cat#:AM1345 | |
| Commercial assay or kit |
MEGAclear Transcription Clean-Up Kit |
Ambion, ThermoFisher Scientific |
Cat#:AM1908 | |
| Chemical compound, drug |
Mecamylamine | Sigma-Aldrich | Cat#:M9020 | 500 μM |
| Software, algorithm | Fiji | NIH | RRID:SCR_002285 | |
| Software, algorithm | R Project for Statistical Computing |
R Foundation | RRID:SCR_001905 | |
| Software, algorithm | Imaris Version 8.0.0 | Bitplane, UK. | RRID:SCR_007370 | |
| Software, algorithm | CATMAID | DOI:10.1093/ bioinformatics/btp266 |
RRID:SCR_006278 | |
| Software, algorithm | PhyML | DOI:10.1093/sysbio/ syq010 |
RRID:SCR_014629 | |
| Software, algorithm | Gblocks | DOI:10.1080/ 10635150701472164 |
RRID:SCR_015945 | |
| Other | Wormglu | GluStitch Inc. | ||
| Other | Fluoresbrite Multifluorescent Microspheres 1.00 µm |
Polysciences Inc, US | Cat#:24062–5 | (1:1000) |