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. 2018 Aug 29;46(22):e132. doi: 10.1093/nar/gky769

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Effects of the stability of hairpin on the cyclization by T4 DNA ligase. (A) The solution conformations of L64_1-4,24-4, L64_1-6,24-4, L64_1-7,24-4 and L60_1-7,20-4, determined by Mfold calculation. (B) Lane 1, L64_1-4,24-4 without T4 ligase treatment; lane 2, L64_1-4,24-4 with T4 ligase treatment; lane 3, L64_1-6,24-4 alone; lane 4, L64_1-6,24-4 with T4 ligase treatment; lane 5, L64_1-7,24-4 alone; lane 6, L64_1-7,24-4 with T4 ligase treatment; lane 7, L60_1-7,20-4 alone; lane 8, L60_1-7,20-4 with T4 ligase treatment. The enzymatic conditions are the same as described in Figure 2.