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. 2018 Aug 29;46(22):e132. doi: 10.1093/nar/gky769

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Comparison of the reaction conversion and yield of monomeric cyclic ring between substrates with and without the terminal hairpin. (A) Time-courses for the T4 ligase-mediated ligation of L64_3-4,24-4 (circles) and L64_16-4,37-4 (rectangles). The total amounts of DNA, consumed in the presence of T4 ligase (by both intramolecular and intermolecular ligation), are plotted as a function of reaction time. In (B), the yield of DNA ring is shown as a function of reaction time. Reaction conditions: [l-DNA]₀ = 5 μM, [splint]₀ = 10 μM, and 10 U T4 DNA ligase in 1× T4 DNA ligase buffer at 25°C.