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(A,B) ABCE1 protein levels in U87 and A172 cells were assessed by Western blot analysis after transfection of ABCE1 shRNA (shABCE1) or negative control shRNA (shNC). (C–F) IC50 and cell viability were determined using the CCK-8 assay after treatment with different concentrations of TMZ. (G,H) The apoptotic rate of U87 and A172 cells was measured by flow cytometry after treatment with TMZ (100 μM). *P<0.05.
