Supplementary Figure S10. Rab (GDP bound) dominant negative mutants do not interact with S1P₁ receptors.

Cells were co-transfected for transient expression of mCherry-tagged S1P₁ receptors and the indicated eGFP-tagged dominant negative Rab proteins. Cells were challenged for the times indicated with 1 μM S1P, 10 μM FTYp, or 1 μM PMA. FRET values were determined and normalized to those observed in the absence of stimulus (Time 0, Baseline; 100 %). In panel A, plotted are the means with vertical lines indicating the S.E.M of 24 samples resulting from four independent experiments, in which six different images were obtained in each of these. Panels B-D, representative images of the experiments expressing the different Rab (GDP) proteins. Other indications as in Supplementary Figure S1.

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