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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Oncogene. 2018 Aug 1;37(50):6414–6424. doi: 10.1038/s41388-018-0419-5

Figure 1. Hippo signaling deficiency induces the cell addiction to YAP. (See also Figure S1).

Figure 1

(A) YAP is activated and accumulated in the nucleus of the LATS1/2 DKO, MOB1A/B DKO and NF2 KO cells. Loss of MST1/2 does not affect YAP cellular localization. Nucleus was visualized by DAPI. Scale bar, 20 μm.

(B) Downregulation of YAP in the Hippo component KO cells. Western blotting was performed with indicated antibodies

(C and D) Loss of YAP specifically suppressed the viability of the LATS1/2 DKO, MOB1A/B DKO and NF2 KO cells. Cell viability was visualized by crystal violet staining (C) and quantified (mean ± s.d., n = 3 biological replicates) (D). * p < 0.05, ** p < 0.01, *** p < 0.001.

(E–G) Loss of YAP but not TAZ suppressed the LATS1/2 DKO cell viability. shRNA-mediated downregulation of YAP and TAZ was confirmed by Western blot in both wild-type HEK293A and LATS1/2 DKO cells (E). Cell viability was visualized by crystal violet staining (F) and quantified (mean ± s.d., n = 3 biological replicates) (G). *** p < 0.001.