Fig. 1.

Effect of antibiotic treatment on metabolism in HFD-fed mice. a Body weight gain of mice on Chow or HFD with or without Abx treatment (metronidazole or vancomycin (n = 10/group). Time 0 is the beginning of the HFD and the dashed line represents the beginning of Abx treatment. b Blood glucose levels in the random fed state of mice on a Chow or HFD with or without Abx treatment (n = 10/group). c OGTT performed in mice on a Chow or HFD with or without Abx treatment (n = 9/group). d AUC of blood glucose levels during an OGTT (n = 9/group). e Weight gain of chow or HFD recipient GF-mice after bacterial transfer from mice treated with chow/HFD with or without Abx (n = 8-9/group). f OGTT of chow or HFD recipient GF-mice 2 weeks after bacterial transfer from mice treated with chow/HFD with or without Abx (n = 8/group). C chow, H HFD, M HFD + metronidazole, V HFD + vancomycin. Data are shown as mean ± SEM. ^##P < 0.01, and ^###P < 0.001 by repeated measure ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001 by ANOVA, followed by pre-planned t-tests