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. 2018 Dec 14;8:17882. doi: 10.1038/s41598-018-36209-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FISH of sex-linked BACs. FISH localizes two markers (E24C3 and E12A6) associated with the sex locus, ambysex, on a DAPI stained metaphase spread of chromosomes from an axolotl embryo of unknown sex. E24C3 is labeled with cy3 (red) and E12A6 is labeled with fluorescein (green). White asterisks show labeling of E24C3, and white arrows point to the labeling of E12A6 on the opposite end of the same chromosomes.