Table 2.
Blast results to nonredundant protein NCBI database.
| Sex-specific Scaffold | Scaffold length (bp) | NCBI Best Hit | Query Cover | E value | % identity | Accession# |
|---|---|---|---|---|---|---|
| SuperContig1084421 | 991 | PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Phaethon lepturus] | 18% | 6.00E-33 | 98% | XP_010292439.1 |
| SuperContig_990642 | 1488 | PREDICTED: transcriptional regulator ATRX isoform X2 [Alligator sinensis] | 17% | 6.00E-13 | 64% | XP_006032758.2 |
| SuperContig_1201750 | 725 | PREDICTED: uncharacterized protein LOC101734340 [Xenopus tropicalis] | 14% | 0.13 | 50% | XP_017945915.1 |
| SuperContig_1270996 | 631 | hypothetical protein [Rhodopirellula baltica] | 12% | 9.7 | 50% | WP_011119337.1 |
| SuperContig_1240926 | 668 | PREDICTED: uncharacterized protein LOC106589496 [Salmo salar] | 39% | 2.00E-16 | 47% | XP_014035031.1 |
| SuperContig_481414 | 11464 | PREDICTED: dynein heavy chain 11, axonemal [Xenopus tropicalis] (reverse transcriptase) | 5% | 1.00E-32 | 43% | XP_017952780.1 |
| SuperContig_1139773 | 843 | aminotransferase class I and II [Streptomyces sp. CB00455] | 17% | 6.2 | 42% | WP_073917349.1 |
| SuperContig_1398497 | 510 | hypothetical protein [Massilia sp. BSC265] | 36% | 4.1 | 40% | WP_051933638.1 |
| SuperContig_1136461 | 850 | flagellar autotomy protein [Micromonas pusilla CCMP1545] (reverse transcriptase) | 12% | 0.55 | 39% | XP_003062983.1 |
| SuperContig_1105317 | 928 | hypothetical protein A2Z37_15870 [Chloroflexi bacterium RBG_19FT_COMBO_62_14] | 12% | 6.3 | 37% | OGO67717.1 |
| SuperContig_960617 | 1857 | PREDICTED: uncharacterized protein LOC106605384 [Salmo salar] | 20% | 1.8 | 36% | XP_014056412.1 |
| SuperContig_446459 | 12684 | ORF2 protein [Salmo salar] (reverse transcriptase) | 8% | 1.00E-36 | 35% | AKP40998.1 |
| SuperContig556195 | 9021 | PREDICTED: uncharacterized protein LOC108708171 [Xenopus laevis] | 19% | 2.00E-70 | 34% | XP_018102087.1 |
| SuperContig_981147 | 1581 | PREDICTED: LOW QUALITY PROTEIN: dynein heavy chain domain-containing protein 1 [Orcinus orca] | 13% | 8.4 | 32% | XP_004279330.1 |
| SuperContig_1025868 | 1238 | DNA primase [Pseudaminobacter manganicus] | 23% | 9.2 | 32% | WP_080921700.1 |
| SuperContig_1035909 | 1185 | hypothetical protein T12_433 [Trichinella patagoniensis] | 16% | 5.5 | 31% | KRY11477.1 |
| SuperContig_1196200 | 734 | DUF948 domain-containing protein [Lactobacillus buchneri] | 41% | 4.7 | 27% | WP_014939867.1 |
The table shows best match amino acid alignments for blast (blastx)78 hit results for all 42 sex-specific scaffolds. 17 scaffolds aligned to a protein-coding gene, and most shared <40% identity. The two highest identity hits to genes were to transcriptional regulator ATRX by SuperContig_990642 and mitogen-activated kinase kinase kinase 2 by SuperContig_108441.