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. 2018 Dec 14;8:17866. doi: 10.1038/s41598-018-36279-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OCTA en face images of four devices. After registration, the four original images of four devices display exactly the same location (upper row). After a process of Hessian filter, auto-threshold and large vessel extraction, we got the binarized images (middle row). The images show single lines of retinal vessels after skeletonization and the selected locations for measurement are overlaid on the skeletonized images (bottom row). The diameters of inner and outer circles are 1.5 mm and 2.25 mm, respectively.