Fig. 3.
Mitochondria are damaged during pyroptosis. a B6Nlrp1b+ BMDMs were preloaded with Mitotracker Red CMXRos and stimulated with LeTx in culture media containing Sytox Green (n = 50). Confocal images were acquired every 3 min. b–e B6Nlrp1b+ BMDMs were preloaded with TMRM and stimulated with either LeTx (b, c) or FlaTox (d, e) and imaged as in (a). Graphs show the percentage of mean fluorescence intensity (MFI) calculated as described in the Methods section, and values represent the mean ± SD of all individual cells that were imaged in five independent experiments (LeTx, n = 28; FlaTox n = 28). Single-cell plots are shown in Supplemental Fig. 3. Fluorescent micrographs show the maximum intensity projection (TMRM and Sytox Green) or the single plane (Mitotracker) of a representative cell. In all panels, time point zero indicates the first detection of Sytox Green. All scale bars, 10 µm