Fig. 5.
Nuclei round up and condense during pyroptosis. a–e B6Nlrp1b+ BMDMs were preloaded with Hoechst dye and stimulated with LeTx (a–c) or FlaTox (c–e) before imaging in culture media containing Sytox Green. Confocal images were acquired every 10 min. Graphs show the percentage of mean fluorescence intensity (MFI) of Sytox Green and nuclear sphericity or the Feret’s diameter based on Hoechst staining, all calculated as described in the Methods section. Values represent the mean ± SD of individual cells imaged in three independent experiments (LeTx: Sphericity n = 24, Feret’s diameter n = 18; FlaTox: Sphericity n = 26, Feret’s diameter n = 20). Single-cell plots are shown in Supplemental Fig. 6. Fluorescent micrographs show the maximum intensity projection of a representative cell. In all panels, time point zero indicates the first detection of Sytox Green. All scale bars, 10 µm