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. 2018 Dec 14;8:17870. doi: 10.1038/s41598-018-35943-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptional and epigenetic analysis in sham, contralateral and IR CKD model. Total RNA and sheared chromatin was prepared from UUO, contralateral (Contra) and sham (Sham) kidneys 24 days after surgeries. Sham IR surgeries were performed in the same manner as the disease model surgeries but without clamping the vascular pedicle,. RT-qPCR RNA and ChIP-qPCR analysis (see Fig. 4). Transcript levels (mRNA) were normalized to L32 mRNA. Matrix ChIP-qPCR analysis of RNA polymerase II (Pol II), and permissive (H3K27Ac, H3K4m3) and repressive (H3K9m2) marks (n = 6 mice from each group). Small solid circle, p < 0.05, large circle, p < 0.01, and no circle, not statistically significant. The results demonstrate that transcript and epigenetic the differences between contralateral and sham kidneys are either small or not detectable. Thus, the contralateral kidneys are appropriate internal controls for this chronic IR model.