Fig. 3.

The cisd-1 gene is expressed in the germline. a Illustration of the cisd-1 locus predicted to produce two isoforms from the cisd-1a and cisd-1b transcripts. CRISPR editing was used to produce a GFP transcriptional reporter strain (PM152) that disrupts the cisd-1 gene. The GFP^SEC^3xFlag sequence was inserted into the second exon of cisd-1b at 536 bp from the start site. b Representative images of L4 cisd-1(pnls27) animals and c 1-day old adult cisd-1(pnls27) animals exhibiting GFP expression in the gonad arm. The N2 wild-type animal is shown to communicate the typical autofluorescence (AFL) observed within the intestine of C. elegans. White arrows indicate transcriptional GFP expression in the germline of cisd-1(pnls27) animals. For b and c, the top panel shows fluorescent images and the bottom panel shows a fluorescent image merged with the DIC image; scale bar = 20 µm. Images are representative animals from 30 animals examined.