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. 2018 Apr 17;26(1):162–178. doi: 10.1038/s41418-018-0108-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The number of gonad cell corpses is reduced in the cisd-1(tm4993) animals with disrupted apoptotic core machinery processes. The number of apoptotic germ cells within the gonad was visualized and quantified, using the ACT-5::YFP reporter strain, for the respective animals: control, ced-3(RNAi), ced-9(n1950gf), cisd-1(tm4993), cisd-1(tm4993); ced-3(RNAi) and cisd-1(tm4993); ced-9(n1950gf)). a Representative images of the gonad arm in respective animals. The ACT-5::YFP reporter is shown individually and merged with the DIC image. White arrows point to the apoptotic cells within the gonad. Scale bar = 20 µm. b The knock-down of ced-3 using RNAi reduced the number of cell corpses in the gonad of cisd-1(tm4993) animals. Relative to control animals, the number of cell death corpses within the gonad was significantly reduced in the ced-3(RNAi) animals, indicating effective RNAi of ced-3. c The ced-9(n1950gf) allele reduced the number of cell corpses in the gonad of cisd-1(tm4993) animals. b, c Identical letters indicate groups with no significant differences; different letters indicate P < 0.05 (Kruskal–Wallis, Dunn’s multiple comparison test). The number of apoptotic corpses within the gonad was quantified in animals from three independent experiments for a total of at least 30 animals. Error bar represents standard deviation.