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. 2018 Dec 6;62:10.29219/fnr.v62.1493. doi: 10.29219/fnr.v62.1493

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© 2018 Xiujing Dou et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

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Fig. 4 — PBA induces HDP gene expression upon histone modification. (a) HDAC activity was determined. The cells were incubated with increasing concentrations of PBA, and TSA was used as a reference. (b) An immunofluorescence analysis of phospho-histone H3 in the IPEC J2 cells, which were treated with PBA and TSA for 24 h. (c) The IPEC J2 cells were transfected with TLR2/4 siRNA before a treatment with PBA, and then HDAC activity was detected. Letters with different superscripts are significantly different at P < 0.01 compared with the vehicle.