Table 1.
List of primers used for qRT-PCR
| Target gene | Sequence (5’-3’) | Reference/accession |
|---|---|---|
| pBD-3 | Forward: GAAGTCTACAGAAGCCAAAT | a |
| Reverse: GGTAACAAATAGCACCATAA | ||
| pEP2C | Forward: GTTGACCTGGGAGCCAAAG | c |
| Reverse: GCACAGATGACAAAGCCTCA | ||
| pBD-1 | Forward: CCGCCTCCTCCTTGTATT | MF925344.1 |
| Reverse: GGTGCCGATCTGTTTCAT | ||
| IL-6 | Forward: TGGCTACTGCCTTCCCTACC | b |
| Reverse: CAGAGATTTTGCCGAGGATG | ||
| IL-8 | Forward: CTGGCTGTTGCCTTCTTG | b |
| Reverse: TCGTGGAATGCGTATTTATG | ||
| IL-18 | Forward: ACTTTACTTTGTAGCTGAAAACGATG | b |
| Reverse: T TT AGG TTC AAG CTT GCC AAA | ||
| TLR2 | Forward: TCACTTGTCTAACTTATCATCCTCTTG | b |
| Reverse: TCAGCGAAGGTGTCATTATTGC | ||
| TLR3 | Forward: AGTAAATGAATCACCCTGCCTAGCA | b |
| Reverse: GCCGTTGACAAAACACATAAGGACT | ||
| TLR4 | Forward: GCCATCGCTGCTAACATCATC | b |
| Reverse: CTCATACTCAAAGATACACCATCGG | ||
| NF-κB1(p50) | Forward: CTCGCACAAGGAGACATGAA | b |
| Reverse: ACTCAGCCGGAAGGCATTAT | ||
| NF-κB3(p65) | Forward: TGTGTAAAGAAGCGGGACCT | c |
| Reverse: CACTGTCACCTGGAAGCAGA | ||
| β–actin | Forward: GGCTCAGAGCAAGAGAGGTATCC | c |
| Reverse: GGTCTCAAACATGATCTGAGTCATCT |
a (14); b (15); c (17); The sequences of pBD-1 in Table 1 are available through GenBank (http://www.ncbi.nlm.nih.gov/nuccore/) under the accession numbers listed above.