Table 1.
Growth rates of Synechococcus 2973 with various SNPs reverted to the allele in Synechococcus 7942
| Nucleotide position | Locus tag | Gene function | Doubling time, h | % change in growth rate |
| 2973-WT | — | Control | 2.1 ± 0.2 | N/A |
| 7942-WT | — | Control | 8.5 ± 1.1 | N/A |
| pUH24 | — | Cure 2973 of plasmid | 2.1 ± 0.0 | 0 |
| 126938 | M744_705 | Hypothetical protein | 2.0 ± 0.0 | 4 |
| 236706* | M744_1335 | ATP synthase F0F1 alpha | 3.1 ± 0.5 | −49 |
| 474883† | — | Noncoding 101 bp upstream of rpaA | 2.1 ± 0.2 | 0 |
| 475352† | M744_2605 | rpaA, SNP I, circadian response regulator | 2.0 ± 0.0 | 4 |
| 475390† | M744_2605 | rpaA, SNP II, circadian response regulator | 2.0 ± 0.0 | 4 |
| 610804 | M744_3335 | Manganese ABC transporter ATP-binding | 2.1 ± 0.2 | 0 |
| 705129 | M744_3855 | Guanylate cyclase | 2.1 ± 0.2 | 0 |
| 891346* | M744_4780 | PpnK – NAD+ kinase | 4.5 ± 0.2 | −117 |
| 1042862 | — | Noncoding, 362 bp upstream of ycfA | 2.1 ± 0.3 | 0 |
| 1080351 | M744_5865 | Hypothetical protein | 2.1 ± 0.0 | 0 |
| 1113358 | M744_6025 | Molecular chaperone DnaK | 2.1 ± 0.5 | 0 |
| 1222741 | M744_6570 | Hydrolase | 2.1 ± 0.2 | 0 |
| 1222972 | M744_6570 | Hydrolase | 2.1 ± 0.2 | 0 |
| 1237531 | M744_6650 | CTP synthetase | 2.1 ± 0.1 | 0 |
| 1238113 | M744_6650 | CTP synthetase | 2.2 ± 0.4 | −4 |
| 1273424 | M744_6850 | Chorismate mutase | 2.5 ± 0.6 | −16 |
| 1533430 | — | Noncoding, 120 bp upstream of COP23 | 2.1 ± 0.3 | 0 |
| 1619501 | M744_8615 | DNA-directed RNA polymerase β subunit | 2.3 ± 0.1 | −9 |
| 1718274 | M744_11685 | Anthranilate synthase | 1.9 ± 0.2 | 7 |
| 1741647 | — | Noncoding, downstream of two genes | 2 ± 0.2 | 4 |
| 1749120 | — | Noncoding, 21 bp upstream of porin | 2.2 ± 0.2 | −4 |
| 2139224 | — | Noncoding, 130 bp upstream of histone like protein | 2.2 ± 0.0 | −4 |
| 2249045 | M744_12130 | Long-chain fatty acid CoA ligase | 2.1 ± 0.1 | 0 |
| 2339502 | M744_12130 | Long-chain fatty acid CoA ligase | 2.0 ± 0.2 | 0 |
| 2347222 | — | Noncoding, 37 bp upstream of hypothetical | 2.2 ± 0.2 | −4 |
| 2348957 | — | Noncoding, 26 bp upstream of hypothetical | 2.1 ± 0.3 | 0 |
| 2347664–2347220 | — | Noncoding, insertion upstream of hypothetical | 2.1 ± 0.3 | 0 |
| 2364720 | M744_12285 | Glutamate synthase | 2.1 ± 0.1 | 0 |
| 2608863 | M744_13540 | Photosystem I assembly protein | 2.0 ± 0.0 | 4 |
| Multiple* | M744_2605/noncoding | rpaA, SNP I SNPII, noncoding promoter SNP combined | 3.4 ± 0.3 | −61 |
Significant difference from Synechococcus 2973 controls: 95% CI (1.87, 2.33). N/A, not applicable.
*
SNPs that significantly slowed growth.
†
SNPs that did not individually slow growth but had an effect when combined.