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SUMOylation of PCNA by PIAS1 and PIAS4 E3 ligases. DT40 cells stably transfected with His-tagged hSUMO1 were analyzed by pull-down assay using Ni-NTA magnetic beads. Whole cell lysates and lysates after pulled down using Ni-NTA magnetic beads are shown on the Left. Quantification of SUMOylated PCNA and unmodified PCNA by pull-down experiments were shown on the Right.
