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. 2018 Dec 14;37:316. doi: 10.1186/s13046-018-0994-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The effect of co-expression linc01296 and miR-26a on CRC progression. a Flow cytometry showed O-linked glycosylation level detected by fluorescein isothiocyanate (FITC)-conjugated VVA on the cell surface of transfected CRC cells. b CCK8 assay showed variant growth rate of transfected CRC cells. c Colony formation assay detected proliferative formation with different treated CRC cells. d Aggressiveness changed was determined by transwell assay, the migratory and invasive cells were counted. e CRC cells were treated with 5-FU, and the cell viability was detected by CCK8 assay. f IC₅₀ values of treated CRC cells were calculated. g The effect of 5-FU on CRC cell proliferation was determined by colony formation assay. h Flow cytometry showed the apoptosis rate of transfected CRC cells in response to 5-FU. i FITC-VVA on transfected CRC cells surface was detected by flow cytometry. j The growth curves of transfected CRC cells were pictured after conducting CCK8 assay. k Proliferation of treated CRC cells was examined by colony formation assay. l Migration and invasion were observed in transfected SW620 cells. m With 5-FU treatment, the cell viability was determined by CCK8 assay. n The IC₅₀ values were subsequently calculated. o 5-FU resistant CRC cells were transfected with linc01296 or miR-26a mimic, and the colony formation was counted in response to 5-FU. p The apoptosis rate of transfected CRC cells was detected after 5-FU treatment by flow cytometry. The data were means ± SD of three independent assays (*P < 0.05)